Dynamic organization of chromosomal DNA in Escherichia coli

Article Abstract:

Dynamic organization of chromosomal DNA in Escherichia coli, organization critical in cell division, chromosome replication, and partitioning in the bacterial cell cycle, is discussed. The subcellular localization of 22 DNA segments of the E. coli chromosome has been studied by the FISH method. The bacterial chromosome is 1000 times the length of the bacterium and folded by some unknown means into a nucleoid, a compact form. The circular chromosomal DNA has been shown to be organized to form a compact ring structure in the cell.

Japan, Usage, Prokaryotes, In situ hybridization, Bacterial genetics, Cytochemistry, Circular DNA

---

Subcellular distribution of actively partitioning F plasmid during the cell division cycle in E. coli

Article Abstract:

F plasmid, the fertility factor of E. coli, is partitioned with fidelity to daughter cells during cell division, due to sopA and sopB, trans-acting genes, and sopC, a cis-acting site. The subcellular distribution of mini-F-plasmid molecules was visualized through fluorescence in situ hyrbidization. Molecules lacking the sopABC segment showed random distribution in spaces not occupied by nucleoids.

Plasmids

---

Polar localization of the replication origin and terminus in Escherichia coli nucleoids during chromosome partitioning

Article Abstract:

Three migration patterns are discernible in the replication origin (oriC), the chromosome terminus and other DNA regions during the partitioning of daughter chromosomes in Escherichia coli. In cell division cycle by FISH, oriC is at the old-pole-proximal and the terminus is at the new-pole-proximal nucleoid borders. OriC, which travel to opposite nucleoid borders, are retained and stay a constant distance from each cell pole.

Similar abstracts:

• Abstracts: Characterization of the minimal replicon of a cryptic Deinococcus radiodurans SARK plasmid and development of a versatile Escherichia coli-D: radiodurans shuttle vectors
• Abstracts: DNA-binding polarity of human replication protein A positions nucleases in nucleotide excision repair. Error-prone bypass of certain DNA lesions by the human DNA polymerase kappa
• Abstracts: Characterization of the replication, maintenance, and transfer features of the IncP-7 plasmid pCAR1, which carries genes involved in carbazole and dioxin degradation
• Abstracts: Molecular interactions continuously define the organizer during the cell movements of gastrulation. The molecular metamorphosis of experimental embryology
• Abstracts: Seasonal variation in diurnal and nocturnal distributions of nonbreeding shorebirds at North Humboldt Bay, California

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.