Error-prone bypass of certain DNA lesions by the human DNA polymerase kappa

Article Abstract:

The human DNA polymerase kappa and its important error-prone bypass of certain DNA lesions are discussed in this research communication. The DinB protein of Escherichia coli is a recently identified error-prone DNA polymerase. A human homolog has been found and named DINB1. The DINB1 gene encodes a DNA polymerase called pol(kappa) and incorporates mismatched bases on an undamaged template often. It bypasses an abasic site and N-2-acetylaminofluorene-adduct in an error-prone way. The human DINB1 in a truncated form allows for the bypass.

Japan, Physiological aspects, DNA damage, Human genetics, Cytogenetics, DNA polymerases

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DNA-binding polarity of human replication protein A positions nucleases in nucleotide excision repair

Article Abstract:

Affinity of human replication protein A (hRPA) for artificial DNA hairpin structures with 3' or 5' protruding single-stranded arms has been compared. Single-stranded DNA (ssDNA) is bound with a defined polarity by hRPA. The human ssDNA-binding replication A protein (RPA) has a role in assorted DNA-processing situations. Its DNA-binding polarity positions shows that nucleases are present in nucleotide excision repair with hRPA on the undamaged strand. It may be that the polarity of hRPA on ssDNA contributes to directionality of other processes dependent on hRPA.

Analysis, Genetic aspects, DNA repair, Molecular biology, Chromosome replication, RNA splicing, DNA binding proteins, Polarity (Biology), Nucleotides, Nucleases, Human chromosomes

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