A soluble two-component regulatory system controls expression of quinoprotein ethanol dehydrogenase (QEDH) but not expression of cytochrome c(sub.550) of the ethanol-oxidation system in Pseudomonas aeruginosa

Article Abstract:

A soluble two-component regulatory system has been found to control expression of quinoprotein ethanol dehydrogenase (QEDH) in Pseudomonas aeruginosa. It does not, however, control expression of cytochrome c(sub.550) of the ethanol-oxidation system. Promoter activity during growth on several substrates was measured using transcriptional lacZ fusions to study regulation of divergent promoters of the exaAB genes in P. aurginosa ATCC 17933. In ATCC17933 exaA encodes a quinoprotein ethanol dehydrogenase and exaB codes for a cytochrome c. Phenotypic characterization and restoration of the wild-type behavior of the different regulatory mutants produced by different subclones and cosmids show that six genes may take part in the regulation of ethanol oxidation in P. aeruginosa.

Bacteria, Genetic regulation, Dehydrogenases, Oxidoreductases, Cytochemistry, Cellular control mechanisms, Cell regulation

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The Pseudomonas aeruginosa acsA gene, encoding an acetyl-CoA synthetase, is essential for growth on ethanol

Article Abstract:

Research has been conducted on the gene acsA encoding acetyl-CoA synthetase. The identification of this gene essential in Pseudomonas aeruginosa ATCC 17933 for the growth on ethanol has been carried out and the results are reported.

Analysis, Carbon, Gene expression, Microbiological research

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Cytochrome C(sub 550) is an essential component of the quinoprotein ethanole oxidation system in Pseudomonas aeruginosa: cloning and sequencing of the genes encoding cytochrome C(sub 55) and an adjacent acetaldehyde dehydrogenase

Article Abstract:

A study was conducted to analyze the function of cytochrome C(sub 550) as an important part of the quinoprotein ethanol oxidation system in Pseudomonas aeruginosa. Mutagenized cells were spread on plates supporting succinate minimal medium to hinder the growth of auxotrophs. Results indicated that a mutant of P. aeruginosa with an interrupted cytochrome c(sub 550) gene failed to grow on ethanol proving the importance of the cytochrome in the ethanol oxidation system.

Alcohol, Ethanol

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