DNA fingerprints by pulsed-field gel electrophoresis to investigate a nosocomial pneumonia caused by 'Legionella bozemanii' serogroup 1

Article Abstract:

A comparison of a patient strain with two environmental strains taken from the hospital water supply indicate that Legionella bozemanii is transmitted from the water to the patient, and acts as a source for nosocomial legionellosis causing nosocomial pneumonia. The strains of L.bozemanii serogroup 1 available from clinical and environmental sources after subtyping with pulsed-field gel electrophoresis, show heterogeneous genomic DNA structure using either the SfiI or NotI restriction enzyme.

Case studies, Observations, DNA testing, DNA identification, Gel electrophoresis, Pneumonia, Nosocomial infections, Cross infection

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Detection of Legionella spp. in cooling tower water by the polymerase chain reaction method

Article Abstract:

A polymerase chain reaction is used to investigate the presence of legionella spp. in cooling tower water. 20-mr 5S rRNA complementary DNA sequence primers detect the total legionella spp., and the specilfic legionella pneumophila is detected with 20-mer and then 21-mer macrophage infectivity potentiator gene sequence primers. Twenty-five were positive for legionella spp., and 14 of the 27 cooling tower water samplescontained L. pneumophila.

Growth, Legionella pneumophila

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Legionella contamination in hot water of Italian hotels

Article Abstract:

A cross-sectional multicenter survey of Italian hotels is conducted to investigate Legionella spp. contamination of hot water. Results reveal that the Italian hotels, particularly those located in old buildings, represent a major source of risk for Legionnaires disease due to the high frequency of Legionella contamination, high germ concentration, and major Legionella pneumophila serogroup 1 colonization.

Italy, Science & research, Surveys, Legionella, Italian restaurants, Legionnaires' disease, Legionellosis

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