Degradation of 2-chloroallyalcohol by a Pseudomonas subspecies

Article Abstract:

The development of a degradation pathway from the microbial growthon 2-chloroallyalcohol is discussed. Cells were harvested from anaerobically grown bacterial strains. A biological oxygen monitor determined alcohol and aldehyde dehydrogenase activities. Capillary gas chromatography quantitatively determined chlorinated allyalcohol and were analyzed by high-pressure liquid chromatography. The suitability of 2-chloroallyalcohol as a substrate for bacterial growth was demonstrated.

Methods, Pseudomonas

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Kinetics of bacterial growth on chlorinated aliphatic compounds

Article Abstract:

The kinetic properties of Xanthobacter autotrophicus GJ10, Ancylobacter aquaticus AD20 and Pseudomonas sp. strain AD1 were measured during growth in continuous cultures and compared to the kinetic properties of the first catabolic enzyme of halogenated aliphatic compound degradation. The results showed that the strains followed Monod kinetics. Correlations between the Monod constants and Km values of the first catabolic enzyme were also observed.

Physiological aspects, Bacterial growth, Halocarbons, Enzyme kinetics

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Discovery of a novel styrene monooxygenase originating from the metagenome

Article Abstract:

The discovery of a new styrene monooxygenase from the metagenome by its ability to form indigo in Escherichia coli, which is able to perform highly enantioselective epoxidation and sulfoxidation reactions, is described.

Analysis, Enantiomers, Alternate generation, Alternation of generations

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