Degradation of 2,4-dichlorophenoxyacetic acid by haloalkaliphilic bacteria

Article Abstract:

The I-18 bacterial isolate from the Alkali Lake, southwestern Oregon, degrades 2,4-dichlorophenoxyacetic acid (2,4-D) by the same pathway as that used by other 2,4-D degrading bacteria. The isolate is a moderately halophilic alkaliphilic bacteria and is adapted to the extreme conditions of the lake. I-18 and two other 2,4-D degrading isolates are members of the Halomonadaceae family, and their 16S rRNA sequences are similar to those of other members. The DNA of the isolates hybridize with the Alcaligenes eutrophus tfdA, tfdB, tfdC, and tfdD genes required for 2,4-D degradation.

Observations, Biodegradation, Identification and classification, Dichlorophenoxyacetic acid, 2,4-D (Herbicide)

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Evidence for interspecies gene transfer in the evolution of 2,4-dichlorophenoxyacetic acid degraders

Article Abstract:

A study was conducted to analyze interspecies gene transfer associated with the evolution of 2,4-dichlorophenoxyacetic acid degraders. A collection of 2,4-D degraders supporting 15 novel strains were obtained by genomic fingerprinting. The degraders were then distributed throughout the alpha, beta, and gamma subgroups of the Proteobacteria. Results indicated incongruency in phylogenies suggesting that 2,4-D degradation originates from gene transfer between species.

Genetic aspects, Phylogeny, Organic acids

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Monitoring of an alkaline 2,4,6-trichlorophenol-degrading enrichment culture by DNA fingerprinting methods and isolation of the responsible organism, haloalkaliphilic Nocardioides sp. strain M6

Article Abstract:

Molecular fingerprinting methods were utilized in the isolation of the M6 strain of Nocardioides sp., a bacterial able to degrade 2,4,6 trichlorophenol. The M6 strain was isolated from an enrichment culture obtained from Alkali Lake, OR. The DNA fingerprinting methods employed in the investigation are extragenic palindromic polymerase chain reaction and amplified ribosomal DNA restriction analysis.

Usage, DNA testing, DNA identification, Microbial biotechnology, Biotechnological microorganisms

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