Genetic and biochemical diversity among isolates of Paenibacillus alvei cultured from Australian honeybee (Apis mellifera) colonies

Article Abstract:

DNA fingerprints of 30 isolates of Paenibacillus alvei were extremely variable and easily discernible. This technique could be used to track the movement of this bacterium, which infects other animals besides honeybees and is also found in soil.

Evaluation, DNA testing, DNA identification, Bacillus (Bacteria), Bacillus

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Serotypes and virulence gene profiles of shiga toxin -producing Escheichia coli strains isolated from feces of pasture-fed and lot-fed sheep

Article Abstract:

The shiga toxin -producing Escherichia coli (STEC) strains contain genes for enterohemolysin (ehxA) and intimin, identified as complex STEC (cSTEC) and are generally recuperated from the feces of human beings with hemolytic uremic and hemorrhagic colitis condition. To identify whether the modifications in the diet affects the excretion pattern of cSTEC, the virulence gene profiles and serotypes of cSTEC were located from pasture-fed sheep.

Science & research, Virulence (Microbiology)

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Detection of Shiga-like toxin (stx1 and stx2), intimin (eaeA), and enterohemorrhagic Escherichia coli (EHEC) hemolysin (EHEC hlyA) genes in animal feces by multiplex PCR

Article Abstract:

Research was conducted to examine a multiplex PCR for the rapid detection of genes encoding Shiga toxins 1 and 2, enterohemolysin and intimin. Data were collected from 444 fecal samples obtained from healthy and clinically affected sheep, goats, cattle and sheep. InstaGene methodology was used to extract nucleic acid from a representative volume of each dilution of the titration. Results demonstrate that the detection limit of the assay ranged from 18 to 37 genome equivalents.

Bacterial toxins, Polymerase chain reaction, Feces

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