Detection of aflatoxigenic molds in grains by PCR

Article Abstract:

The use of selected genes, present in the aflatoxin biosynthesis pathway, in the PCR is effective in the detection of aflatoxigenic molds inhabiting stored grains. The ver-1, omt-1 and apa-2 are the genes involved in the aflatoxin biosynthetic pathway. The amplification occurs only in the DNA of the Aspergillus parasiticus and Aspergillus flavus in all the primer pairs of the three genes. The inoculation of sterile corn with other molds fails to amplify the DNA even at the highest inoculum level.

Usage, Grains, Microbiological synthesis, Polymerase chain reaction, Grain

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Purification of a 40-kilodalton methyltransferase active in the aflatoxin biosynthetic pathway

Article Abstract:

The conversion of sterigmatocystin (ST) to O-methylsterigmatocystin (OMST) is the last level in the aflatoxin bosynthetic pathway of the filamentous fungi Aspergillus flavus and A. parasiticus. Five soft-gel chromatographic steps were used to carry out the purification of the reaction catalyst, S-adenosylmethionine-dependent methyltransferase. A second enzyme that can convert ST to OMST in vitro was characterized from the purification process.

Biosynthesis, Methyltransferases

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