Detection of hemolysin variants of Shiga toxin-producing Escherichia coli by PCR and culture on vancomycin-cefixime-cefsulodin blood agar

Article Abstract:

Restriction fragment length polymorphisms of polymerase chain reaction products were used to investigate the presence of hemolysin-encoding gene (elyA) in different Shiga toxin-producing Escherichia coli (STEC) serovars. Amino acid and nucleotide sequences of the STEC serovars were similar to those obtained from reference strains. Different enrichment broths were used to identify the ability of STEC strains in stool and food specimens. Repression of gene expression may have influenced the differences observed in hemolysin detection.

Usage, Polymerase chain reaction, Gene expression

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Epidemiological relatedness and clonal types of natural populations of Escherichia coli strains producing Shiga toxins in separate populations of cattle and sheep

Article Abstract:

Two separate animal populations composed of a herd of cattle and a flock of sheep were studied for strains of Escherichia coli producing Shiga toxins (STEC) for a period of six months. Thirty-three STEC were isolated from 63.2% of cattle and grouped into 11 serotypes and eight electrophoretic types by multilocus enzyme analysis. Eighty-eight percent of the sheep excreted STEC belonging to 17 different serotypes and 12 different electrophoretic types.

Microbial toxins

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Identification of human-pathogenic strains of shiga toxin-producing Escherichia coli from food by a combination of serotyping and molecular typing of shiga toxin genes

Article Abstract:

Shiga toxin producing Escherichia coli (STEC) strains form meat, milk and cheese samples were investigated for their serotypes and for genetic variants of Shiga toxins 1 and 2 (STx1 and Stx2). Typical enterohemorrhagic Escherichia coli (EHEC) strains were found rarely among STEC isolates from food, whereas atypical EHEC strains belonging to known human-pathogenic types were detected frequently.

Genetic aspects, Shigella

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