Retention of enteropathogenicity by viable but nonculturable Escherichia coli exposed to seawater and sunlight

Article Abstract:

Enterotoxigenic strains of Escherichia coli H10407 retain pathogenicity even after being exposed to sunlight, and entering the viable but nonculturable state. When exposed to sunlight, the direct counts of viable cells fail to change. Escherichia coli cells are culturable in the absence of sunlight only when exposed to seawater. Ganglioside-enzyme-linked immunosorbent assay (GM1-ELISA) shows that Escherichia coli continues to produce enterotoxin after exposure to sunlight. GM1-ELISA is found to be superior to the conventional rabbit intestinal loop assay.

Observations, Escherichia coli, Solar radiation, Marine microbiology

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Molecular epidemiological survey of rotaviruses in sewage by reverse transcriptase semitested PCR and restriction fragment length polymorphism assay

Article Abstract:

Southern blot hybridization combined with reverse transcription and polymerase chain reaction amplification of the VP7 gene was utilized to detect rotavirus contamination in raw sewage and treated effluent samples. Restriction fragment length polymorphism (RFLP) of the amplification products indicated the presence of a variety of rotavirus dsRNAs belonging to human and animal rotavirus sequences. Furthermore, the treated effluent samples yielded ten different RFLPs of human and animal origin.

Identification and classification, Polymerase chain reaction, Medical microbiology, Rotaviruses

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Recovery in embryonated eggs of viable but nonculturable Campylobacter jejuni cells and maintenance of ability to adhere to HeLa cells after resuscitation

Article Abstract:

Research conducted on three human isolates of pathogenic bacteria Campylobacter jejuni to investigate their ability to recover culturability after egg passage is analyzed. Results demonstrate that viable but not culturable C. jejuni cells maintain adhesion potential after the passage.

International, Physiological aspects, Cell adhesion, Human embryo, Microbiological research

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