Development of a universal diluting fluid for membrane filtration sterility testing

Article Abstract:

A univeral diluting fluid (UDF), based on the Dey-Engley neutralizing broth, was developed for the inactivation of a broad range of preservatives in order to enhance microbial recovery in membrane filters. The UDF contained a combination of inactivating agents at concentrations minimizing inherent toxicity. Tests on its neutralizing effectiveness against several common preservative agents showed that UDF is useful in sterility testing. The broad efficacy eliminates the need for multiple diluting fluids for each preservativeclass during sterility testing.

Innovations, Sterilization (Disinfection), Sanitary microbiology, Sterilization

---

Comparison of membrane filtration and multiple-tube fermentation by the colilert and enterolert methods for detection of waterborne coliform bacteria, Escherichia coli, and enterococci used in drinking and bathing water quality monitoring in southern Sweden

Article Abstract:

The detection of Escherichia coli and enterococci in Swedish drinking and bathing water through membrane filtration and multiple-tube fermentation by the Colilert and Enterolert methods have been compared. A total of 261 drinking water samples and 77 bathing water samples were analyzed twice using routine Swedish procedures. Results indicate that the Colilert method is more sensitive than the Swedish methods in detecting coliform bacteria. It also has equal sensitivity in detecting E. coli when all drinking water samples were grouped together.

Methods, Analysis, Escherichia coli, Enterobacteriaceae, Microbiology, Drinking water, Microbiological assay

---

A rapid, specific membrane filtration procedure for enumeration of enterococci in recreational water

Article Abstract:

The EPA recommends the use of a two-step membrane filter method with mE medium to measure enterococci, which serves as an indicator of fecal contamination, in fresh and marine recreational waters. A study was conducted to assess the changes to the commercially available base medium mE agar, to examine the specificity of the modified medium for enteroccoci, and to evaluate differences among laboratories using the modified medium for samples taken from different aquatic environments. The findings are discussed.

Research, Aquatic microbiology, Membrane filters, Membrane separation

Similar abstracts:

• Abstracts: Development of a chemically defined medium for the growth of Leuconostoc mesenteroides. Effects of pH and trace minerals on long-term starvation of Leuconostoc mesenteroides
• Abstracts: Comparative evaluation of modified m-FC and m-TEC media for membrane filter enumeration of Escherichia coli in water
• Abstracts: Instability of a novel multicolored flower trait in inbred and outcrossed soybean lines. FISH mapping of the 5S and 18S-28S rNDA loci in different species of Glycine
• Abstracts: Envelope glycoproteins of human immunodeficiency virus type 1: profound influences on immune functions. Sexual agglutination in budding yeasts: structure, function, and regulation of adhesion glycoproteins
• Abstracts: Induction mechanism of a single gene molecule: stochastic or deterministic? Transgenic animal studies on the evolution of genetic regulatory circuitries

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.