Development of an immunomagnetic bead-immunoliposome fluorescence assay for rapid detection of Escherichia coli O157:H7 in aqueous samples and comparison of the assay with a standard microbiological method

Article Abstract:

The study aims to develop and optimize a protocol for the rapid detections of Escherichia coli O157:H7 in aqueous samples by a combined immunomagnetic bead-immunoliposome (IMB/IL) fluorescence assay. The use of this procedure is expected to increase the speed by which E. coli O157:H7 might be identified in aqueous specimens linked to epidemiological investigation and might thus increase the speed by which health care facilities and the population at large might be alerted of possible health threats.

Pharmaceutical Preparation Manufacturing, Pharmaceutical preparations, Biological Product (except Diagnostic) Manufacturing, Biological products exc. diagnostic, Liposomes, Liposome Drug Delivery Systems, Escherichia coli

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Microbiological transformations of lipids: acyl-specific hydrolysis of lard by yeasts

Article Abstract:

The result of lard hydrolysis by cultures of Saccharomyces cerevisiae and Schizosaccharomyces octosporus is discussed. Gas-liquid chromatography was sued to determine fatty acid production. The positional specifities of the lipases of both organisms were determined by synthetic triglycerides. Results indicate that valuable and usable pure fatty acids can be biotranformed to produce yeast hydrolytic enzymes.

Research, Usage, Yeast, Yeast (Food product), Lipid research, Hydrolysis, Lard

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A model to esimate the optimal sample size for microbiological surveys

Article Abstract:

A statistical model based on a Bayesian mode of inference for the estimation of the sample size for an accurate analysis of bacterial subtypes in a specimen. The model gives probability distribution of strains in a specimen that is used to estimate the probability of identifying all strains present in the sampled colonies.

Models, Statistics, Statistics (Data), Statistical sampling, Sampling (Statistics), Microbiological research

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