Development of bacterium-based heavy metal biosorbents: enhanced uptake of cadmium and mercury by Escherichia coli expressing a metal binding motif
Article Abstract:
A study was conducted to analyze the preparation of a gene coding for a de novo peptide sequence supporting a metal binding motif. The gene was expressed in Escherichia coli as a fusion with the maltose binding protein. Heavy metal removal by cells expressing the peptide was examined. Experimental results indicated that the metal binding peptide fusion supported enhanced binding of Cd2 and H2+ than bacterial cells with no metal binding peptide.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Involvement of an extracellular H2O2-dependent ligninolytic activity of the white rot fungus Pleurotus ostreatus in the decolorization of Remazol brilliant blue R
Article Abstract:
The white rot fungus Pleurotus ostreatus exhibits H2O2-dependent ligninolytic activity of Remazol brilliant blue R (RBBR) oxygenase. Cysteine, NaN3, and NaCN inhibit activity of the enzyme. Therefore, the enzyme seems to have a catalytic metal center, probably heme. The enzyme shows maximal activity between pH 3.5 and 4. It is not dependent on manganese and veratryl alcohol. The enzyme functions also in the absence of RBBR.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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Influence of cadmium and mercury on activities of ligninolytic enzymes and degradation of polycyclic aromatic hydrocarbons by Pleurotus ostreatus in soil
Article Abstract:
Research has been conducted on the white-rot fungus Pleurotus ostreatus. The ability of this fungus to degrade polycyclic aromatic hydrocarbons has been investigated.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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