Monitoring impact of a pesticide treatment on bacterial soil communities by metabolic and genetic fingerprinting in addition to conventional testing procedures

Article Abstract:

Research was conducted to examine the impacts of a pesticide treatment on bacterial soil communities taking herbogil, a reference herbicide, the mineral oil Oleo and Goltix as model compounds. The effects on metabolic sum parameters were established through the monitoring of substrate-induced respiration and dehydrogenase activity and nitrogen and carbon mineralization. The BIOLOG system and pattern generation by amplified ribosomal sequences were used to analyze the suitability of the approaches for the analysis of the impacts of herbicide applications.

Bacteria, DNA testing, DNA identification, Microbial pesticides

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Phylogenetic analysis of an anaerobic, trichlorobenzene-transforming microbial consortium

Article Abstract:

A new hybridization-based screening method for bacterial 16S rDNA clone libraries is used in studying an anaerobic, trichlorobenzene-transforming microbial community. Small-subunit rRNA genes were amplified using polymerase chain reaction from community DNA by using primers specific for Bacteria or Euryarchaeota and the genes were subsequently cloned. The new screening approached detected 51 bacterial clone families and one clone family was closely related to the dechlorinating Dehalobacter species.

Methods, Environmental aspects, Genetic aspects, Nucleic acid hybridization, Anaerobic bacteria, Chlorobenzene, Chlorobenzenes

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Peptide nucleic acid-mediated PCR clamping as a useful supplement in the determination of microbial diversity

Article Abstract:

Results demonstrate that polymerase chain reaction clamping technique can be employed in the analysis of microbial diversity in complex microbial communities. Data indicate that the technique functions as a supplement to the statandard polymerase chain reaction amplification in ribosomal-DNA based studies.

Germany, Statistical Data Included, Analysis, Usage, DNA, Polymerase chain reaction, Microbial ecology

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