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Development of monoclonal antibodies that identify Vibrio species commonly isolated from infections of humans, fish, and shellfish

Article Abstract:

Monoclonal antibodies (MAbs) were developed against Vibrio alginolyticus, V. anguillarum, V. carchariae, V. cholerae, V. damsela, V. furnissii, V. harveyi, V. ordalii, V. parahaemolyticus and V. vulnificus for use in rapid identification of these bacteria. Serological experiments showed that some of the MAbs cross-reacted with closely-related Vibrio species, indicating a common antigenic determinant among them. Three MAbs recognized nearly all of the Vibrio species, which again suggests a common epitope among them. The complementary use of genus-specific and species-specific MAbs is a rapid method for identifying different Vibrio species.

Author: Smith, A., Chen, D., Hanna, P.J., Altmann, K., Moon, P., Hammond, L.S.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
Vibrio, Antigenic determinants

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Epitope specificity and application of Salmonella typhimurium O-antigen-specific monoclonal antibodies

Article Abstract:

The use of anti-O:5 antigen antibodies developed by Drs. Jerzy Zawitowski and Z.W. Jaradat for the detection of Salmonella typhimurium will only lead to false negatives and positives during the serological process. The monoclonal antibodies are not effective in detecting Salmonella typhimurium because O:5 antigen expression is rare in these pathogens. Furthermore, serogroup-specific monoclonal antibodies coupled with enzyme-linked immunoabsorbent assay is more effective in the serological identification of Salmonella typhimurium.

Author: Luk, John M., Tsang, Raymond S.W.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
Analysis, Bacteria, Salmonella typhimurium, Serology

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Rapid determination of members of the family Enterobacteriaceae in drinking water by an immunological assay using a monoclonal antibody against enterobacterial common antigen

Article Abstract:

A rapid and highly specific immunological method for detecting Enterobacteriaceae in drinking water was developed. The procedure involved the detection of an enterobacterial common antigen via the monoclonal antibody G2a 898 using a sandwich ELISA method. Evaluation of the procedure showed that it is faster and more reliable than the conventional methods used for detecting microbial contamination in drinking water.

Author: Hubner, I., Steinmetz, I., Obst, U., Giebel, D., Bitter-Sueramann, D.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
Enterobacteriaceae, Water, Aquatic microbiology, Microbial contamination, Sanitary microbiology

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Subjects list: Research, Usage, Identification and classification, Monoclonal antibodies, Methods
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