Development of primers to O-antigen biosynthesis genes for specific detection of Escherichia coli O157 by PCR

Article Abstract:

A study has revealed that DNA sequences in Salmonella rfb operons have identified regions that are conserved and regions that are unique to a given Salmonella serovar. The conservation of these rfb gene sequences were believed to be reflective of the presence of common sugars on O antigens in some members of the Enterobacteriaceae family. A polymerase chain reaction test conducted on Escherichia coli O157 serotypes revealed that as few as 10 cells with the specific rfb oligonucleotide primers were present.

Usage, Escherichia coli, Polymerase chain reaction, Gene expression, Bacterial antigens, O antigens

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Dissemination of fluoroquinolone-resistant Campylobacter spp. within an integrated commercial poultry production system

Article Abstract:

Commercial broiler hatching eggs, which were incubated in a research facility until the chicks hatched, were obtained to determine whether the presence of Campylobacter DNA in young chicks was due to ingestion of the bacteria in food or water. The results have shown that resistant Campylobacter coli can be present in multiple levels of an integrated poultry system and molecular methods or more sensitive culture techniques might be required to detect early colonization by Campylobacter in broiler chicks.

Science & research, Pharmaceutical Preparation Manufacturing, Pharmaceutical preparations, Quinolone Antibiotics, Broiler, fryer, and roaster chickens, Chickens, Broilers, Broilers and Other Meat Type Chicken Production, Diseases, Genetic aspects, Drug resistance in microorganisms, Microbial drug resistance, Campylobacter, Quinolone antibacterial agents, Fluoroquinolones, Broilers (Poultry), Broilers (Chickens), Quinolones, Chemical properties

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