Development of a genetic system for Geobacter sulfurreducens

Article Abstract:

Researchers show that Geobacter sulfurreducens can fix nitrogen by disrupting the nifD gene, which is involved in nitrogen fixation in other organisms. This means the bacterium would be able to bioremediate petroleum contaminated environments, which are carbon-rich but nitrogen-poor.

Petroleum, Biodegradation, Bioremediation, Nitrogen-fixing microorganisms

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Direct correlation between rates of anaerobic respiration and levels of mRNA for key respiratory genes in Geobacter sulfurreducens

Article Abstract:

The direct correlation between the respiration rates and the mRNA levels of key respiratory genes in Geobacter sulfurreducens are determined with the help of fumarate as the electron acceptor and acetate as the limiting electron donor in anaerobic continuous cultures. The results show that mRNA levels of respiratory genes are used to estimate in situ Fe(III) reduction rates in Geobacter-dominated environments.

Messenger RNA, Microbiological research

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DNA microarray analysis of nitrogen fixation and Fe(III) reduction in Geobacter sulfurreducens

Article Abstract:

A DNA microarray representing the genome of Geobacter sulfurreducens is developed for use in global gene expression profiling of cells under steady-state conditions with acetate as the electron donor and Fe(III) as the electron acceptor. A substantial proportion of significantly expressed genes during Fe(III) reduction were genes of unknown function or hypothetical proteins, suggesting differences in Fe(III) reduction physiology among microorganism that perform this metabolic process.

United States, Analysis, Nitrogen, Nitrogen fixation, DNA microarrays

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