Directed evolution of pyruvate decarboxylase-negative Saccharomyces cerevisiae, yielding a C(sub)2-independent, glucose-tolerant, and pyruvate-hyperproducing yeast

Article Abstract:

Research describes production and characterization of pyruvate decarboxylase-negative strains of Saccharomyces cerevisiae capable of growing in presence of high concentraions of glucose as the carbon and energy source. Also, described is obtaining a C(sub)2-independent pyruvate decarboxylase-negative strains of Saccharomyces cerevisiae by progressively decreasing acetate in the culture medium.

Manufacturing processes, Science & research, Research, Production processes, Microbiological synthesis, Nutritional aspects, Pyruvates

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Overproduction of threonine aldolase circumvents the biosynthetic role of pyruvate decarboxylase in glucose-limited chemostat cultures of Saccharomyces cerevisiae

Article Abstract:

Results show that overexpression of threonine aldolase enables pyruvate decarboxylase-negative mutant of Saccharomyces cerevisiae to grow under carbon limiting conditions in chemostat cultures containing glucose. Data suggest that acetaldehyde formed by threonine aldolase initiates acetyl-CoA synthesis, which is usually the case in the wild-type cells.

Carbohydrate metabolism, Enzyme regulation

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Engineering of Saccharomyces cerevisiae for efficient anaerobic alcoholic fermentation of L-arabinose

Article Abstract:

Fast and efficient anaerobic alcoholic fermentation of L-arabinose by an engineered Saccharomyces cerevisiae strain is described. The resulting Saccharomyces cerevisiae strain exhibited high rates of arabinose consumption and ethanol production and a high ethanol yield during anaerobic growth on L-arabinose as the sole carbon source.

Research and Development in the Physical, Engineering, and Life Sciences, Genetic Engineering, Analysis

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