Early days of gel electrophoresis

Article Abstract:

A geneticist recalls his early experiments with gel electrophoresis and explains how he discovered that starch could be heated and cooled to provide an electrophoretic medium for testing serum proteins. His technique enabled him to gain a deeper understanding of molecular genetics at the protein level, which he extended to duplicate mouse genes to analyze genetic traits. The starch gel electrophoretic method was developed into the two-dimensional electrophoresis, which helps resolve more than 750 protein spots in serum.

Analysis, Gel electrophoresis

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Ultraviolent radiation screening compounds

Article Abstract:

The evolution of methods to attenuate ultraviolet (UV) radiation has long been a ubiquitous problem for life, especially for photosynthetic organisms that rely on solar radiation for their energy needs. UV screening compounds have well-defined groups throughout a wide range of organisms. A UV-screening compound is a passive process and an experimentally observed screening should be physiologically relevant.

Ultraviolet radiation

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Screening for carriers of the avian cartilage proteoglycan core protein defect, nanomelia, by denaturing gradient gel electrophoresis

Article Abstract:

Nanomelia, a lethal mutation among birds, is characterized by the absence of a core protein for a proteoglycan required in cartilage synthesis. A novel method identifying carriers of the trait is presented. This new method utilizes the different electrophoretic behavior of mutant DNA corresponding to nanomelia. The technique compares favorably with carrier-detecting standard progeny phenotype tests.

Genetic aspects, Birds, Lethal mutation

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