Polyvinylpyrrolidone-agarose gel electrophoresis purification of polymerase chain reaction-amplifiable DNA from soils

Article Abstract:

A rapid and simple method for the purification of polymerase chain reaction amplifiable DNA from soil is provided by a modification of agarose gel electrophoresis. Polyvinylpyrrolidone is added to the agarose gel by this modification. The electrophoretic mobility of denaturing phenolic compounds is retarded by the addition of polyvinylpyrrolidone, and they do not comigrate with nucleic acids.

Innovations, Genetic aspects, Gel electrophoresis

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Reaction of acylated homoserine lactone bacterial signaling molecules with oxidized halogen antimicrobials

Article Abstract:

Oxidized halogen compounds can block acylated homoserine lactone bacterial signaling, which is used by bacteria residing in biofilms. This might prevent biofilm formation on surfaces. However, only acylated homoserine lactones with a 3-oxo group react with oxidized halogen compounds.

Prevention, Microbial mats, Cellular signal transduction

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Bacterial production of D-erythroascorbic acid and L-ascorbic acid through functional expression of Saccharomyces cerevisiae D-arabinono-1,4-lactone oxidase in Escherichia coli

Article Abstract:

Research was conducted to produce D-arabino-1,4-lactone oxidase involved in the biosynthesis of vitamin C variant in Escherichia coli. The data show that the expressed enzyme produced vitamin C and its variant in high quantities.

South Korea, Research, Methods, Recombinant DNA, Yeast fungi, Microbial biotechnology, Yeasts (Fungi), Vitamin C

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