Pooling DNA in the identification of parents

Article Abstract:

The preliminary pooling of DNA samples to cut down on the number of tests necessary to identify which of a set of potential parents could be the parent of a plant offspring is discussed, as are designs with each parent in multiple pools and sequential use of as many as three independent marker loci. Best pool sizes are calculated for a range of marker allele frequencies and numbers of potential parents. The progeny may be homo- or heterozygous at the marker locus and the marker alleles of the other parent plant are not necessarily known.

United Kingdom, Usage, Genetic screening, Genetic testing, Genetic markers, Experimental design, Research design, Sex in plants, Sex (Botany)

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RAPD reveals genetic similarity of Acer saccharum and Acer nigrum

Article Abstract:

North American sugar maples were first characterized more than 100 years ago, but the number of species and subspecies has since been a matter of dispute, particularly with regard to the distinction between black maples and sugar maples, Acer nigrum and A. saccharum. Now RAPD has revealed genetic similarity of the two and it seems distinctive characteristics may be from a relatively small gene set or be inducible by local environment.

Biological diversity, Biodiversity, Sugar-maple, Sugar maple, Maple, Maples

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Spatial genetic structure of Delphinium nuttallianum populations: inferences about gene flow

Article Abstract:

Results show less marked genetic allozymic differentiation among six populations of Delphinium nuttallianum spread over 3 km. Data indicate that postpollination selection may have an influence on the gene flow in the spatial genetic make-up of plant populations.

Analysis, Observations, Spatial behavior, Human spatial behavior, Inbreeding, Gene frequency, Delphinium, Delphiniums

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