Effect of O-side-chain-lipopolysaccharide chemistry on metal binding

Article Abstract:

Research was conducted to examine the effect of O-side-chain-lipopolysaccharide chemistry on metal binding. To locate and analyze metal deposition sites, transmission electron microscopy with energy-dispersive X-ray spectroscopy was used while atomic absorption spectrophotometry and inductively coupled plasma-mass spectrometry were used to conduct bulk quantitation of bound metal. Results indicate that the binding of metallic ions is not caused by the negatively charged sites located in the O-side chains.

Endotoxins, Pseudomonas aeruginosa, Metal ions

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Mixed-valence cytoplasmic iron granules are linked to anaerobic respiration

Article Abstract:

The intracellular granules containing ferric and ferrous iron formed in Shewanella putrefaciens CN32 during dissimilatory reduction of solid-phase ferric iron is reported. It is the first in situ detection at high resolution of a mixed-valence metal particle residing within a prokaryotic cell of Fe(III) reduction which might indicate a respiratory role.

Science & research, Prokaryotes, Anaerobic respiration

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Sorption of Fe (hydr)oxides to the surface of Shewanella putrefaciens: cell-bound fine-grained minerals are not always formed de novo

Article Abstract:

The attachment of Fe (hydro)oxides to Shewanella putrefaciens does not always involve de novo growth. Large amounts of ferrihydrite, goethite, and hematite bind to the bacterium and may even penetrate the outer membrane.

Bacteria, Bacterial adhesion, Iron compounds

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