Microvariation artifacts introduced by PCR and cloning of closely related 16S rRNA gene sequences

Article Abstract:

Research is presented on tracking artifactual sequence variation sources within 16S rRNA clone libraries. A polymerase chain reaction method was applied to a defined template mixture containing seven 16S-rRNA clones that are closely related; artifact sources found included a mutational hot spot, polymerase errors and heteroduplex and chimera cloning.

United Kingdom, United States, Netherlands, Statistical Data Included, Methods, Usage, Genetic aspects, Polymerase chain reaction, Bacterial genetics, Cloning, Microbial populations, Errors, Scientific, Scientific errors

---

Effect of toxic metals on indigenous soil beta-subgroup proteobacterium ammonia oxidizer community structure and protection against toxicity by inoculated metal-resistant bacteria

Article Abstract:

The effect of treating soil with a mixture of toxic metals on the population structure and size of the ammonia oxidizers that are members of the beta subgroup of the Proteobacteria family has been studied. These metals are cadmium, cobalt, cesium, strontium and chlorides. The addition of five strains of metal-resistant heterotrophic bacteria to the soil has also been examined. Results revealed that the addition of the metals brought specific changes in the community structure of the ammonia oxidizers which were not seen with the metal-resistant bacteria.

Physiological aspects, Environmental aspects, Soil pollution, Soils, Heavy metals

---

Molecular analysis of ammonia-oxidizing bacteria of the beta subdivision of the class Proteobacteria in compost and composted materials

Article Abstract:

A molecular analysis of ammonia-oxidizing bacteria of the beta subdivision of the class Proteobacteria in compost and composted materials was conducted. The objective was to examine the distribution and community composition of the oxidizers in different types of composting materials by amplifying 16S ribosomal DNA and 16S rRNA using PCR and reverse transcriptase PCR, respectively. Results revealed the presence of nucleic acids from nitrifying bacteria in the materials which may have substantial implications for compost processing and storage.

Compost, Nitrogen-fixing microorganisms

Similar abstracts:

• Abstracts: Corresponding between community structure and function during succession in phenol- and phenol-plus-trichloroethene-fed sequencing batch reactors
• Abstracts: Numerical analysis of grassland bacterial community structure under different land management regimens by using 16S ribosomal DNA sequence data and denaturing gradient gel electrophoresis
• Abstracts: Characterization of a cultural alphaproteobacterial symbiont common to many marine sponges and evidence for vertical transmission via sponge larvae
• Abstracts: Surface interactions between Escherichia coli and hemocytes of the Mediterranean mussel Mytilus galloprovincialis Lam. leading to efficient bacterial clearance

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.