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Effects of agronomic treatments on structure and function of ammonia-oxidizing communities

Article Abstract:

Researchers found no significant differences in the diversity of ammonia-oxidizing microorganisms in soils treated with different agricultural treatments. They used denaturing gradient gel electrophoresis and oligonucleotide probing to analyze 16S rRNA genes.

Author: Dollhopf, Sherry L., Phililps, Carol J., Harris, Dave, Gross, Katherine L., Prosser, James I., Paul, Eldor A.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
Agricultural bacteriology, Bacteriology, Agricultural

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Numerical analysis of grassland bacterial community structure under different land management regimens by using 16S ribosomal DNA sequence data and denaturing gradient gel electrophoresis

Article Abstract:

Denaturing gradient gel electrophoresis analysis of PCR amplification products can be used to identify complex microbial populations in soil. This technique was used to analyze microbial populations in native and improved grasslands.

Author: Prosser, James I., Glover, L. Anne, McCaig, Allison E.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
Measurement, Gel electrophoresis, Microbial populations

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Comparative diversity of ammonia oxidizer 16S rRNA gene sequences in native, tilled, and successional soils

Article Abstract:

A study was conducted to compare the autotrophic ammonia oxidizer population structures in long-term ecological research soils using polymerase chain reaction amplification of microbial community DNA with primers specific for 16S rRNA genes. The comparative study was done to assess the effects of disturbance on certain soil bacteria. Findings revealed that tilled soils contained higher population sizes than native culturable soils.

Author: Paul, Eldor A., Kowalchuk, George A., Stephen, John R., Bruns, Mary Ann, Prossier, James I.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
Polymerase chain reaction, Ammonia

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Subjects list: Research, Soil microbiology, Usage
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