Effects of granule-associated protein PhaP on glycerol-dependent growth and polymer production in poly(3-hydroxybutyrate)-producing Escherichia coli

Article Abstract:

The effect of PhaP, a phasin, on bacterial growth and the accumulation of poly(3-hydroxybutyrate) (PHB) from glycerol in bioreactor cultures of recombinant Escherichia coli carrying phaBAC from Azotobacter sp. strain FA8 are examined. The behavior of recombinant Escherichia coli strains carrying phasin genes in a bioreactor is useful in the design of bacterial strains sufficient for the efficient production of polyhydroxyalkanoates (PHAs) from glycerol.

Genetic aspects, Observations, Escherichia coli, Microbial growth

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Microbial conversion of glycerol to 1, 3-propanediol: Physiology comparison of a natural producer, Clostridium butyricum VPI 3266, and an engineered strain, Clostridium acetobutylicum DGI (pSPD5)

Article Abstract:

A study is conducted on the comparative physiological behavior of the recombinant Clostridium bacetobutylicum DG1(pSPD5) strain with that of the natural 1,3-propanediol producer Clostridium butyricum VPI 3266, by growing both the strains in chemostat cultures with glycerol as the sole carbon source. It is observed that both the strains showed the same 'global behavior'.

Analysis, Physiological aspects, Microbiological synthesis, Clostridium botulinum, Nucleic acids, Chemical properties, Nucleic acid isolation

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