Effects of growth medium, inoculum size, and incubation time on culturability and isolation of soil bacteria

Article Abstract:

The aim of the study was to investigate the effects of growth medium, inoculum size, colony density, and incubation time on the appearance of colonies of members of these poorly studied groups of soil bacteria on plates of solid growth media inoculated with soil. The successful isolation of members of groups of bacteria that are widely distributed and common in soils worldwide seems to be a result of using appropriate media and extended incubation times.

Microbial populations

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Detection and cultivation of soil Verrucomicrobia

Article Abstract:

A simple approach that has generated a collection of new strains of the phylum Verrucomicrobia is reported. The analysis has shown that colony development by members of the class 'Spartobacteria' (subdivision of the phylum Verrucomicrobia) is inhibited by other culturable bacteria.

United States, Drugs, In-Vitro Diagnostic Substance Manufacturing, Bacterial Culture Products, Bacteriology, Bacterial cultures

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