Effects of iron limitation on the degradation of toluene by Pseudomonas strains carrying the TOL (pWWO) plasmid

Article Abstract:

Low levels of iron in soil can reduce the biodegradation of hydrocarbons because most microbial hydrocarbon degradation pathways involve iron-containing oxygenases. This was demonstrated in Pseudomonas putida mt2 and P. putida WCS358, both of which have a plasmid that codes for enzymes involved in toluene degradation.

Iron compounds

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Degradation of toluene and trichloroethylene by Burkholderia cepacia G4 in growth-limited fed-batch culture

Article Abstract:

A study was conducted to observe the trichloroethylene (TCE) degradation capacity of Burkholderia cepacia (B. cepacia) G4 in a growth-limited fed-batch culture. Toluene was used as the primary substrate and was also added through the gas phase, like TCE. Results showed that B. cepacia is capable of stably degrading TCE under nongrowth conditions. However, TCE triggered an elevation in the toluene demand per amount of cells of B. cepacia G4 maintained in the culture.

Cell culture, Tissue culture

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Effect of trichloroethylene on the competitive behavior of toluene-degrading bacteria

Article Abstract:

A study was conducted to assess the influence of trichloroethylene (TCE) on toluene-degrading bacterial strains such as Pseudomonas putida mt-2, P. putida F1, P. putida GJ31, and Burkholderia cepacia G4. The strains were placed in a mixed culture at similar numbers when TCE was absent. The addition of TCE resulted in a ninefold reduction in the affinity for toluene of the three disappearing strains which only connotes toluene degradation by TCE. There was also less meta-cleavage activity.

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