Enhanced reactivity of Rhizopus oryzae lipase displayed on yeast cell surfaces in organic solvents: Potential as a whole-cell biocatalyst in organic solvents

Article Abstract:

Applications of genetically immobilized Rhizopus oryzae lipase displayed on the cell surface of Saccharomyces cerevisiae in organic solvents and measured the catalytic activity of the displayed enzyme as a fusion protein with alpha-agglutinin is evaluated. The results show that the yeast cells displaying the lipase can be used in organic solvents and the lipase activity may be increased by a combination of protein engineering and display techniques.

Rice, Rice Farming, Physiological aspects, Genetic aspects, Yeast fungi, Yeasts (Fungi), Lipoprotein lipase

---

In vivo synthesis of mammalian-like, hybrid-type N-glycans in pichia pastoris

Article Abstract:

An engineering of the P. pastoris N-glycosylation pathway to produce nonhyperglycosylated hybrid glycans are described. This is done by inactivation of OCH1 and over expression of an alpha-1,2-mannosidase retained in the endoplasmic reticulum and N-acetyglucosaminyltransferase I and beta-1,4-hybrid-type N-linked oligosaccharide structure on its glycoproteins.

Endoplasmic reticulum, Microbiology, Glycosylation, Transferases

---

Similar abstracts:

• Abstracts: Genetic diversity of Xanthomonas oryzae pv. oryzae in Asia. Novel candidate virulence factors in rice pathogen Xanthomonas oryzae pv. oryzicolas as revealed by mutational analysis
• Abstracts: Spread of recombinant DNA by roots and pollen of transgenic potato plants, identified by highly specific biomonitoring using natural transformation of an Acinetobacter sp
• Abstracts: Ascending migration of endophytic rhizobia, from roots to leaves, inside rice plants and assessment of benefits to rice growth physiology
• Abstracts: pH Gradient-induced heterogeneity of Fe(III)-reducing microorganisms in coal mining-associated lake sediments
• Abstracts: Detection of Salmonella spp. and Listeria monocytogenes in suspended organic waste by nucleic acid extraction and PCR

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.