Enumeration and cell cycle analysis of natural populations of marine picoplankton by flow cytometry using the nucleic acid stain SYBR Green I

Article Abstract:

A flow cytometry method utilizing the nucleic acid stain SYBR Green I is effective for cell enumeration and cell cycle analysis of natural populations of picoplankton in oceanic environments. SYBR Green I distinguishes heterotrophic bacteria and autotrophic Prochlorococcus cells, unlike the TOTO-1 and TO-PRO-1 stains. It also distinguishes the two groups of heterotrophic bacteria. The DNA distribution resolution obtained with SYBR Green I is the same as that obtained with Hoechst 33342. It also allows the analysis of photosynthetic prokaryotes over the whole water column.

Methods, Observations, Nucleic acids, Marine plankton, Microbiological assay

---

Application of the novel nucleic acid dyes YOYO-1, YO-PRO-1, and picogreen for flow cytometric analysis of marine prokaryotes

Article Abstract:

Flow cytometric analyses are conducted for cultures of Escherichia coli and a variety of marine prokaryotes using novel blue light-excited fluorescent dyes for nucleic acid. Results suggest that the dyes are very sensitive to ionic strength, making them unsuitable for cell cycle analysis in natural samples. However, the dyes are very useful for enumerating prokaryotic cells by flow cytometry.

Escherichia coli, Materials, Dyes and dyeing, Dyeing, Dyes

---

Cell cycle regulation by light in Prochlorococcus strains

Article Abstract:

Light appears to trigger DNA replication and cell division in Prochlorococcus strains. In total darkness, cells were arrested in the G(sub 1) phase and in the S phase.

Physiological aspects, Microorganisms, Cell cycle, Light

Similar abstracts:

• Abstracts: Induction and apoptotic regression of lung adenocarcinomas by regulation of a K-Ras transgene in the presence and absence of tumor suppressor genes
• Abstracts: Purification and characterization of mSin3A-containing Brg1 and hBrm chromatin remodeling complexes. Mitotic inactivation of a human SWI/SNF chromatin remodeling complex
• Abstracts: Genetic population history relationships of the population of Bogota, Colombia, by using the D1S80, VWA, and TH01 molecular markers
• Abstracts: Comparative 16S rRNA analysis of lake bacterioplankton reveals globally distributed phylogenetic clusters including an abundant group of actinobacteria
• Abstracts: Histone H2A.Z regulates transcription and is partially redundant with nucleosome remodeling complexes. Histone acetyltransferase complexes stabilize SWI/SNF binding to promoter nucleosomes

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.