Purification and characterization of mSin3A-containing Brg1 and hBrm chromatin remodeling complexes

Article Abstract:

The establishment of cell lines that express epitope-tagged Brg1 and hBrm and characterization of the complexes associated with the two ATPases are described. Brg1 has been found to fractionate into two complexes different in subunit composition and activity, but hBrm is seen in cone complex with lower activity than the Brg1 complexes. Unexpected functional characteristics show the hSWI/SNF complexes under consideration rave diverse regulatory functions. Evidence that the Brg1 and hBrm complexes have in them subunits of the human Sin3 complex has been seen.

Statistical Data Included, Genetic aspects, Human beings, Histones, Humans, Adenosine triphosphatase

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Mitotic inactivation of a human SWI/SNF chromatin remodeling complex

Article Abstract:

In mitosis, chromatin condenses into mitotic chromosomes. Transcription is inhibited. Those processes might be opposed by the chromatin remodeling of the SWI/SNF complexes. Human SWI/SNF (hSWI/SNF) complexes have been purified at different stages in the cell cycle, and hSWI/SNF was not active in cells blocked in G2-M. It appears that a transitional inactivation and reactivation of hSWI/SNF is necessary for forming of a repressed chromatin structure in mitosis and reforming of an active chromatin structure as cells go out of mitosis.

Observations, Genetic regulation, Gene expression, Genetic transcription, Transcription (Genetics), Mitosis, Phosphorylation

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ATP-dependent remodeling and acetylation as regulators of chromatin fluidity

Article Abstract:

ATP-dependent acetylation and remodeling are discussed as regulators of chromatin fluidity in a review article. Topics considered include the order in which the remodeling complexes and acetyltransferases act in relation to each other and to transcription processes. Experiments carried out on ATP-dependent remodeling complexes are summarized. A model suggesting the main role of the complexes is creating a fluid chromatin structure is proposed. It is proposed that the fluid chromatin is locking an activated or repressed state by acetylation/deacetylation complexes with other factors.

Adenosine triphosphate, ATP

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