Estimation of methanogen biomass by quantitation of coenzyme M

Article Abstract:

The extreme oxygen sensitivity of methanogenic bacteria and inherent limitations of culturing techniques make it difficult to accurately find a biomass value. The thiol biomarker coenzyme M (CoM), known to be in all methanogenic bacteria, is used. A high-performance liquid chromatography (HPLC)-based method for detecting thiols in pore water was modified to quantify CoM in pure cultures, sewage water or sediment. CoM derivative identity was verified through use of liquid-chromatography-mass spectroscopy.

United States, Statistical Data Included, Usage, Measurement, Biomass, Methanobacteriaceae, Methanogens, Molecular microbiology, High performance liquid chromatography

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Characterization of two subsurface H2-utilizing bacteria, Desulfomicrobium hypogeium sp. nov. and Acetobacterium psammolithicum sp. nov., and their ecological roles

Article Abstract:

The abundance of acetogenic bacteria was found in subsurface sandstone and shale deposits during the Cretaceous period. This prompted scientists to study the terminal steps of the microbial food chain in anaerobic subsurface communities. Originally, these subsurface communities were thought to consume H2 and acetate in the same manner as their surface-borne counterparts. Most types of acetogenic bacteria have complex growth requirements and these processes are enhanced by the addition of glucose.

Physiological aspects, Bacteria, Bioremediation, Microbial ecology

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A rapid and simple method for estimating sulfate reduction activity and quantifying inorganic sulfides

Article Abstract:

A simplified passive extraction methodology for measuring reduced inorganic sulfur compounds from sediments and water is shown. This technique may also be employed for the estimation of sulfate reduction rates. Efficient extraction of FeS, FeS2 and S2- was accomplished with this method. However, the efficiency for S0 depended on the form that was tested. Comparison of the passive extraction technique with the established single-step method produced comparable results with less effort.

Analysis, Sulfur compounds

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