Specific detection of Legionella pneumophila: construction of a new 16S rRNA-targeted oligonucleotide probe

Article Abstract:

The oligonucleotide probe LEGPNE1 was developed using comparative sequence analysis. It aims to detect the pathogen Legionella pneumophila by complementing a variable region in the 16S ribosomal RNA of the pathogen. To test the probe's efficacy, in situ hybridization was conducted in four serogroups of six L. pneumophila strains, five Legionella species as well as non-legionella species. Results indicate that LEGPNE1 was an effective detection tool for L. pneumophila in pure cultures as well as following amoebic infection.

Research, Nucleotide sequence, Base sequence, Identification and classification, DNA probes, Ribosomal RNA

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Resuscitation of viable but nonculturable Legionella pneumophila Philadelphia JR32 by Acanthamoeba castellanii

Article Abstract:

The population of Legionella pneumophila strain Philadelphia I JR 32 that was suspended in sterilized tap water was quantified to determine the intracellular environment that promotes the replication of the microbe. Incubation of Legionella pneumophila JR 32 in tap water microcosms induced a viable but nonculturable (VBNC) state after 125 days. However, the dormant bacteria was resuscitated from the VBNC state after the inoculation of the tap water microcosm with Acanthamoeba castellanii.

Methods, Analysis, Gram-negative bacteria, Bacteriology, Bacterial cultures

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Mycobacterium avium bacilli grow saprozoically in coculture with Acanthamoeba polyphaga and survive within cyst walls

Article Abstract:

Mycobacterium avium is a water-borne human pathogen. Coculture of M. avium with Acanthamoeba polyphaga in mixed cultures and in parachambers reveal that M. avium can grow on substrates secreted by A. polyphaga and does not need actual contact with the amoeba for growth. Further, electron microscopic studies of cysts from infected Acanthamoeba polyphaga show that M. avium lodges inside the outer wall of the cysts during periods unfavorable to growth.

Environmental aspects, Cultures (Biology), Amoeba, Amoebas, Mycobacterium avium

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