Staphylococcal surface display of metal-binding polyhistidyl peptides

Article Abstract:

Researchers have succeeded for the first time in producing recombinant strains of Staphylococcus that display metal-binding polyhistidyl peptides on their surface. These bacteria could be used in bioremediation of heavy metals and could also be used as biosensors.

Bioremediation, Recombinant proteins

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Fluorescence-activated cell sorting of specific affibody-displaying staphylococci

Article Abstract:

Research has been conducted on Staphylococcus carnosus display system. The autrhors report the use of genetically engineered domains to test the functionality of the display system for the target cell enrichment from the background cell excess via the use of flow cytometry.

Research and Development in the Physical, Engineering, and Life Sciences, Sweden, Genetic Engineering, Usage, Physiological aspects, Environmental aspects, Genetic aspects, Cells (Biology), Microbiology, Fluorescence, Gene expression, Cells, Microbial populations, Microbial ecology

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Evaluation of staphylococcal cell surface display and flow cytometry for postselectional characterization of affinity proteins in combinatorial protein engineering applications

Article Abstract:

The staphylococcal cell surface display along with flow cytometry is used for the affinity characterization of candidate affibody molecules directly on the cell surface. The results have shown that staphylococcal surface display has many advantages over phase display, not only in the actual selection progress but also in the time-consuming downstream characterization part.

Technology application, Protein engineering

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