Selective and sensitive method for PCR amplification of Escherichia coli 16S rRNA genes in soil

Article Abstract:

Results demonstrate that the method for PCR amplification discriminates Escherichia coli from other enteric bacteria. Data point out that the technique takes less than 2 hours and effectively detects E. coli DNA in heterogeneous DNA samples extracted from soil.

United States, Statistical Data Included, Methods, Analysis, Measurement, DNA, Escherichia coli, Polymerase chain reaction, Soil chemistry

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Cloning, nucleotide sequencing, and functional analysis of a novel, mobile cluster of biodegradation genes from Pseudomonas aeruginosa strain JB2

Article Abstract:

Researchers report a cluster of genes in Pseudomonas aeruginosa strain JB2 that can degrade hydroxy- and halo-aromatic compounds. The resulting proteins appear to be a ring hydroxylating oxygenase, an ATP-binding cassette-type transporter, an extradiol ring-cleavage dioxygenase, transcriptional regulatory proteins, and chlorocatechol degrading enzymes.

Genetic aspects, Biodegradation

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Integration of matrix-assisted laser desorption ionization-time of flight mass spectrometry and molecular cloning for the identification and functional characterization of mobile ortho-halobenzoate oxygenase genes in Pseudomonas aeruginosa strain JB2

Article Abstract:

A mobile element that carries a gene for ortho-1,2-halobenzoate dioxygenase appears to be globally distributed in some Pseudomonas aeruginosa strains. The gene was found in strains in California, USA, and in Russia.

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