Evaluation of the Duopath Legionella lateral flow assay for identification Legionella pneumophila and Legionella species culture isolates

Article Abstract:

Duopath Legionella (Merck KGaA, Darmstadt, Germany) is a new immunochromatographic assay for the simultaneous identification of cultured L. pneumophila and Legionella species other than L. pneumophila. In tests of 89 L. pneumophila strains and 87 Legionella strains other than L. pneumophila representing 41 different species, Duopath and a widely used latex agglutination assay detected L. pneumophila with 100% and 98% accuracy respectively, whereas the percentages differed significantly for other Legionella spp.

Methods, Bacterial genetics, Chromatography

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Identification and differentiation of Legionella pneumophila and Legionella spp. with real-time PCR targeting the 16S rRNA gene and species identification by mip sequencing

Article Abstract:

A sensitive and specific real-time PCR assay capable of identifying Legionella pneumophila and simultaneously differentiating it from other non-L. pneumophila species by melting curve analysis is established by employing fluorescent resonance energy transfer probes targeting the 16S rRNA gene. A procedure for identification of non-L. pneumophila spp. based on mip sequencing is also presented.

Gene mutations, Gene mutation, Identification and classification, Ribosomal RNA

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Temperature-regulated formation of mycelial mat-like biofilms by Legionella pneumophila

Article Abstract:

Fifty strains representing 38 species of the genus Legionella were examined for biofilm formation on glass, polystyrene and polypropylene surfaces in static cultures at various temperatures. Strains of Legionella pneumophila were found to have the highest ability to form biofilms among the test strains.

Microbial mats

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