Evidence for the adhesive function of the exopolysaccharide of Hyphomonas strain MHS-3 in its attachment to surfaces

Article Abstract:

Hyphomonas cells which form the exopolysaccharide (EPS) capsule are capable of binding to their substratum, while those cells that do not form the EPS capsule cannot do so. Periodate treatment and binding of Bauhinia purpurea lectin and Calcofluor to the EPS reduces the adhesive capacity of the EPS capsule while pronase treatment does not have any effect. This shows that EPS is an adhesin and also forms the biofilm matrix. The exact mechanism of EPS adhesion is not known and it is possible that other molecules are also capable of the adhesive function.

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Spatial and temporal deposition of adhesive extracellular polysaccharide capsule and fimbriae by Hyphomonas strain MHS-3

Article Abstract:

Research was conducted to demonstrate that adhesive fimbriae and capsular exopolysaccharides (EPS) are expressed both temporally and polarly by the Hyphomonas strain MHS-3, a member of the marine bacterial genus that colonizes surfaces immersed in marine water. Another objective was to show that the presence and location of these structures correlate with the previously reported timing and locus of cell adhesion. Results indicate that the timed synthesis and placement of the capsule and fimbriae coincidded with the locus and timing of MHS-3 adhesion.

Cell adhesion molecules

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Production and characterization of monoclonal antibodies specific for Shewanella colwelliana exopolysaccharide

Article Abstract:

The production and characterization of monoclonal antibodies (MAbs) specific to the exopolysaccharide (EPS) of Shewanella colwelliana were reported. Aco1, -4 to -6, -22 and -23 bound to both the cell surface and the purified EPS, while Aco2 and -3 bound to cells only. All of the anti-EPS MAbs bound to the same epitope. Depyruvalation, deacetylation and periodate oxidation had no effect on MAb binding, suggesting that terminal nonreducing sugars were not required for antigenicity.

Analysis, Monoclonal antibodies

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