Evolution of a pathway for chlorobenzene metabolism leads to natural attenuation in contaminated groundwater

Article Abstract:

Various microbial isolation and genetic techniques were used to determine whether chlorobenzene (CB) metabolism is a natural microbial response to contamination with synthetic chemicals. Results indicate that the ability to degrade CB is not an inherent property, but one which has evolved. The evolution is a result of simple horizontal gene transfer and gene recombination between different ancestral strains.

Research, Microbial metabolism, Genetic recombination, Chlorobenzene, Chlorobenzenes

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Unusual location of two nearby pairs of upstream activating sequences for HbpR, the main regulatory protein for the 2-hydroxybiphenyl degradation pathway of 'Pseudomonas azelaica' HBP1

Article Abstract:

Transcriptional fusions between deleted versions of hbpR-hbpC intergenic region and the bacterial luciferase genes demonstrate that most of the transcriptional output from the hbpC promoter is mediated from the proximal upstream activating sequences C-1/C-2. Furthermore, if C-1/C-2 are deleted, C-3/C-4 are appropriately placed with respect to the promoter.

Genetic aspects, Pseudomonas, Evolution (Biology), Genetic regulation, Genetic transcription, Transcription (Genetics), Evolution, Protein metabolism

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Measurement of biologically available naphthalene in gas and aqueous phases by use of a Pseudomonas putida biosensor

Article Abstract:

Research demonstrates that bacterial biosensors react reliably to naphthalene exposure with a bioluminescence signal that is proportional to the concentration of naphthalene. These sensors can be used in chemostat cultivation environment and in gas and liquid phases. The design and construction of the biosensors are discussed.

Product information, Naphthalene, Petrochemical Manufacturing, Measurement, Testing, Environmental aspects, Design and construction, Biosensors, Bioluminescence, Chemostat

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