Expression of active human tissue-type plasminogen activator in Escherichia coli

Article Abstract:

A study was conducted to show that engineering the disulfide bond machinery of the cell through the expression of periplasmic cysteine oxidoreductases supported the production of active full-length tissue plasminogen activator (tPA). Results indicated several ways for increasing the expression of active, full-length tPA. Findings also correlated the oxidative folding of eukaryotic proteins in the periplasmic space with the isomerization of mismatched disulfides.

Analysis, Cells (Biology), Cells, Tissue plasminogen activator, Cysteine

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The LDL receptor-related protein LRP6 mediates internalization and Lethality of Anthrax toxin

Article Abstract:

A demonstration that low density lipoprotein receptor related protein 6 (LRP6), known to be a coreceptor for the Wnt signaling pathway, is required for anthrax toxin lethality in mammalian cells is presented. The results reported indicate that the LRP6 protein, which was known principally as a mediator of the Wnt signaling pathway, has a hitherto unsuspected role in the delivery of anthrax toxins into cells.

Cell research, Cytological research, Anthrax, Low density lipoprotein receptors

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RraA: a protein inhibitor of RNase E activity that globally modulates RNA abundance in E. coli

Article Abstract:

Research demonstrates that the 17.4 kilodalton regulator of ribonuclease activity A, RraA, binds to ribonuclease E and inhibits the latter's endonucleolytic cleavages. Data indicate that RraA abundance circumvents an autoregulatory mechanism that maintains the cellular concentration of ribonuclease E leading to a genome-wide accumulation of ribonuclease E-targeted transcripts.

Physiological aspects, Messenger RNA, Ribonuclease, Enzyme regulation

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