Expression of green fluorescent protein in streptococcus gordonii DL1 and its use as a species -specific marker in coadhesion with streptococcus oralis 34 in saliva-conditioned biofilms in vitro

Article Abstract:

Research points out that bacteria expressing a green fluorescent protein are preferable to fluorescently labeled probes in investigations requiring species-specific markers. Data show that green fluorescent protein expression by oral Streptococcus gordonii DL1 variants can serve as a species-specific markers for oral biofilm analysis.

Analysis, Usage, Measurement, Physiological aspects, Bacteria, Microbial mats, Fluorescence, Bacterial adhesion, Gene expression, Fluorescent antibody technique

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Altered adherence properties of Streptococcus gordonii hppA (oligopeptide permease) mutant result from transcriptional effects on cshA adhesin gene expression

Article Abstract:

Chloramphenicol acetyltransferase gene reporter is fused with csh A gene promoter to examine the regulation of expression of cshA. Test results indicate that HppA production influences the transcription of the cshA gene and the expression of related adherence properties. In addition, cshA promoter is observed to be active during the late exponential phase of growth as seen from measurements of cat mRNA levels and CAT enzyme activities.

Streptococcus, Cell adhesion

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Influence of different functional elements of plasmid pGT232 on maintenance of recombinant plasmids in Lactobacillus reuteri populations in vitro and in vivo

Article Abstract:

The influence of different functional elements of plasmid pGT232, which is indigenous in Lactobacillus reuteri 100-23, on maintaining recombinant plasmids in L. reuteri populations in vitro and in vivo is discussed. The plasmid replicates via the rolling-circle mechanism. The reconstituted lactobacillus-free (RLF) mouse is the experimental animal model for the preliminary studies. Stable maintenance of the pGR232-derived plasmids in the lactobacillus population in vivo required an additional 1.0-kb sequence having a putative single-strand replication origin (SSO). The SSO of pGT232 is throught to be novel and acts in an orientation-specific manner.

Statistical Data Included, Genetic aspects, Lactobacillus, Plasmids, Cytochemistry

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