Expression of the mosquitocidal toxins of Bacillus sphaericus and Bacillus thuringiensis subsp. israelensis by recombinant Caulobacter crescentus, a vehicle for biological control of aquatic insect larvae

Article Abstract:

The 51.4- and 41.9-kDa toxins from Bacillus sphaericus 2297, the 100-kDa toxin from B. sphaericus SSII-1 and the 130-kDa toxin from B. thuringiensis subsp. israelensis were cloned into the broad-host-range plasmid pRK248. The recombinant plasmids were transformed intoCaulobacter crescentus CB15, and toxin expression was achieved. High levels of expression were achieved by fusing the genes to the lacI-regulated tac promoterof pJK5. Toxicity tests on mosquitoes showed that the recombinant C. crescentuscells were toxic to mosquito larvae.

Microbial genetic engineering, Microbial insecticides

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New gene from nine Bacillus sphaericus strains encoding highly conserved 35.8-kilodalton mosquitocidal toxins

Article Abstract:

The mtx3 gene of Bacillus sphaericus is an open reading frame that encodes the Mtx3 mosquitocidal toxin. The gene is highly conserved and is present in strains producing both low- and high-toxicity mosquitocidal toxins. Mtx3 contains 326 amino acids and has a molecular mass of 35.8 kDa. Mtx3 is structurally similar to toxins produced by B. sphaericus SSII-1, Clostridium perfringens and Pseudomonas aeruginosa.

Analysis, Bacillus (Bacteria), Bacillus, Amino acid sequence, Amino acid sequencing

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Transcriptional analysis of the toxin-coding plasmid pBtoxis from Bacillus thuringiensis subsp. israelensis

Article Abstract:

A survey of the transcription of genes with predicted functions was carried out to understand the possible role of pBtoxis in its host bacterium Bacillus thuringiensis subsp. israelensis. The result indicates that plasmids, like pBtoxis, encode not only the toxins that are directly responsible for insect pathogenicity but also a variety of the host organism.

United States, Science & research, Plasmids, Host-bacteria relationships, Chemical properties

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