Expression of the phospho-beta-glycosidase ArbZ from Lactobacillus delbrueckii subsp. lactis in Lactobacillus helveticus: substrate induction and catabolite repression

Article Abstract:

Research reveals that the Lactobacillus helveticus transformed with the arbZ gene confers the ability to use arbutin and cellobiose, salicin and methyl-beta-glucoside by the bacteria. Data point out that arbZ is a phospho-beta-glyosidase enzyme and its catabolite repression is brought about by glucose, mannose, fructose and galactose.

United States, Statistical Data Included, Enzymes, Genetic regulation, Glucose metabolism, Enzyme regulation

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Lactobacillus delbrueckii subsp. lactis DSM7290 pepG gene encodes a novel cysteine aminopeptidase

Article Abstract:

New peptidase genes of Lactobacillus delbrueckii namely pepC and pepG which encode cysteine aminopeptidase and cysteine protease respectively were identified. The activities of these two genes were compared using beta-NA and p-nitroanilide substrates and leucine or proline-containing peptides applied in a Salmonella typhimurium growth test. Findings revealed that the enzymes have different substrate specificities even though they are structurally related.

Aminopeptidases

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PepR1, a CcpA-like transcription regulator of Lactobacillus delbrueckii subsp. lactis

Article Abstract:

Research was conducted to examine whether the open reading frame, pepR1, encodes a catabolite-control protein, CcpA, in a thermophilic bacterial species. Results demonstrate that pepR1 possesses CcpA-like functions and modulates gene transcription which, in the presence of lactose, causes a twofold increase in the prolidase activity.

Research, Observations, Plasmids, Bacteria, Thermophilic, Fermentation, Bacterial genetics, Microbial mutation, Thermophiles

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