Factors affecting exocellular polysaccharide production by Lactobacillus delbrueckii subsp. Bulgaricus grown in a chemically defined medium

Article Abstract:

Researchers developed a chemically defined medium (CDM) that could be used instead of milk for producing exopolysaccharides from Lactobacillus delbrueckii, subsp. Bulgaricus. The bacterium produced as much EPS in CDM as it did in milk and the proportions of monosaccharides were similar in both media.

Culture media (Biology), Culture media

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Optimization of exopolysaccharide production by Lactobacillus delbrueckii subsp. bulgaricus RR grown in a semidefined medium

Article Abstract:

A response surface experimental design was used to examine the optimal fermentation temperature, pH and Bacto-casitone concentration required by Lactobacillus delbrueckii subsp. bulgaricus to produce exopolysaccharide (EPS) in a semidefined medium. Under optimum conditions, which entailed a temperature of 38 degrees Centigrade, pH 5.0 and Bacto-casitone concentration of 30 gram per liter, it was found that the actual yield was 354 mg of EPS per liter.

Physiological aspects, Microbiological chemistry

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Enhancement of exopolysaccharide production by Lactobacillus delbrueckii subsp. bulgaricus NCFB 2772 with a simplified defined medium

Article Abstract:

Research was conducted to examine the medium requirements for growth and production of exopolysaccharides by Lactobacillus delbrueckii subs bulgaricus NCFB 2772. The bacterial strain was grown in batch cultures using the single omission of medium components method to determine its nutritional requirements. Results demonstrated that the strain required only calcium pantothenate, riboflavin and nicotinic acid as individual vitamins. Lesser growth was observed when only these vitamins were present but, in the complete medium, the amount of exopolysaccharide became greater.

Bacterial growth, Bacteriology, Bacterial cultures

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