Factors affecting the determination of respiratory activity on the basis of cyanoditolyl tetrazolium chloride reduction with membrane filtration

Article Abstract:

Membrane filtration and incubation of bacteria on fluorochorme cyanoditolyl tetrazolium chloride (CTC) are the preliminary steps for the evaluation of bacterial respiratory activity. The incubated cells are stained with 4'6-diamidino-2-phenylindole and the respiring cells are counted by epifluorescence microscopy. An increase in pH or phosphate concentration decreases the CTC reduction by Klebsiella pneumoniae, Salmonella typhimurium and Escherichia coli. Respiration of K. pneumoniae is unaffected by an increase in nutrients.

Respiration, Membrane separation

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Legionella confirmation using real-time PCR and SYT09 is an alternative to current methodology

Article Abstract:

A colony-based confirmatory assay that uses real-time PCR and a double-stranded-DNA-intercalating dye, SYTO9 is described for the rapid identification of Legionella pneumophila and Legionella spp. This new assay helped in saving cost and time and also allowed the culture, confirmation and serogrouping of Legionella pneumophila in as few as 3 days.

Science & research, Bacterial genetics, Legionella

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Rapid staining and enumeration of small numbers of total bacteria in water by solid-phase laser cytometry

Article Abstract:

This article describes a rapid staining technique, using the nucleic acid stain SYBR Green I, for enumerating bacterial cell counts in water samples. Results indicate that the method is superior to R2A agar plate method in that the cell counts are obtained within 30 minutes as opposed to 14 days.

United States, Methods, Usage, Bacteria, Stains and staining (Microscopy), Stains (Microscopy), Cell research, Cytological research

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