Flow cytometry detection of infectious rotaviruses in environmental and clinical samples

Article Abstract:

An indirect immunofluorescence and flow cytometry (IIF-FC) method was developed to identify infectious rotaviruses from fecal or water samples. CaCO2 cell-line and ulterior FC were utilized to recognize infected cells because of the cell line's sensitivity to rotavirus infection. Acetone, methanol and formalin combinations were used as fixatives because of their ability to provide superior specific/signal background ratio. The technique offers several advantages such as reproducibility and the ability to process large number of samples.

Diagnosis, Flow cytometry, Immunofluorescence, Fluorescent antibody technique, Rotavirus infections, Rotaviruses

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Development, evaluation, and standardization of a real-time TaqMan reverse transcription-PCR assay for quantification of hepatitis A virus in clinical and shellfish samples

Article Abstract:

A standardized real-time reverse transcription-PCR (RT-PCR) assay was developed for an accurate estimation of the number of genome copies of hepatitis A virus (HAV) in clinical and shellfish samples. The best performance in terms of sensitivity and reproducibility was achieved by a one-step TaqMan RT-PCR, with the sensitivity enabling the detection of 0.05 infectious unit and 10 copies of a single-stranded RNA (ssRNA) synthetic transcript and the test proved to be highly specific after a broad panel of enteric viruses was tested.

Analysis, Polymerase chain reaction, Microbial genetics

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Development and evaluation of a broadly reactive TaqMan assay for rapid detection of hepatitis A virus

Article Abstract:

Primers and a TaqMan probe for the 5' -untranslated region (UTR) of the hepatitis A virus (HAV) genome were designed and evaluated. The assay detected 0.5 infectious units of HAV and 40 copies of a synthetic transcript and provided an important screening tool for rapid quantitative HAV detection in clinical or environmental samples.

Environmental aspects, Biological assay, Bioassay

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