Improvement of oligonucleotide probe design criteria for functional gene microarrays in Environmental applications

Article Abstract:

The application of microarrays to environmental samples presents many technical challenges, a major one being the need to specifically and reliably distinguish between homologous genes from many different organisms that may share a high degree of sequence similarity. To optimize oligonucleotide probe design criteria, PCR products with different similarities to probes were hybridized to a functional gene microarray designed to detect homologous genes from different organisms.

Nucleotide sequence, Base sequence, Genetic research

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Microarray-based analysis of subnanogram quantities of microbial community DNAs by using whole-community d

Article Abstract:

A whole-community genome amplification-assisted microarray detection approach was developed based on multiple displacement amplification for analyzing microbial community structure and its application was demonstrated to low-biomass groundwater microbial communities. The representatives of the amplification were evaluated with the help of several types of microarrays and quantitative indexes.

Analysis, Polymerase chain reaction, Bacterial genetics, Genomic imprinting

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