Frameshifting in the expression of the E. coli trpR gene occurs by the bypassing of a segment of its coding sequence

Article Abstract:

The Escherichia coli trpR gene can be expressed through two reading frames, resulting in the synthesis of two possible gene products. Reporter gene fusion experiments showed that this frameshifting occurs through a bypass of a large segment of the coding sequence. Primer extension analysis showed that this bypass is not pretranslational and probably occurs at the translational level. The bypassing event requires the presence of a specific trpR sequence which is preceded by a nonspecific open reading frame longer than 10 translatable codons at the 5' end.

Codon, Codons

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How do bacteria decide where to divide?

Article Abstract:

Septal ingrowth in Escherichia coli is determined by a division inhibitor and a topological specificity factor encoded in the minC, minD and minE genes. The minC and minD genes cooperate to form a division inhibitor that blocks the formation of septa in the other division sites. The MinE protein lends the topological specificity to the region not covered by the action of minC/minD. Non-expression of minC or minD produces minicell formation (cells without the chromosomal DNA), while non-expression of minE produces non-septate filaments.

Bacterial genetics, Cell division

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A role for a small stable RNA in modulating the activity of DNA-binding proteins

Article Abstract:

Molecular evidence suggests that an RNA molecule in Escherichia coli, 10Sa RNA, plays an important role by modulating the action of DNA-binding proteins during translation. Gel mobility assays showed that 10Sa RNA changes the interaction of DNA-binding proteins with their DNA targets by binding repressors and by competing with the proteins for binding to cognate sites. The mechanism by which 10Sa RNA performs this function is discussed.

RNA, DNA binding proteins

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