GcvA, a LysR-type transcriptional regulator protein, activates expression of the cloned Citrobacter freundii ampC beta-lactamase gene in Escherichia coli: cross-talk between DNA-binding proteins

Article Abstract:

Molecular cloning and characterization of an Escherichia coli gene, gcvA, reveal that expression of the Citrobacter freundii ampC beta-lactamase gene in Escherichia coli is promoted by the gcvA gene product and this promotion is independent of the normal activator AmpR. GcvA is a Lysr-type transcriptional regulator protein as revealed by the amino acid sequence. GcvA binds to the AmpR binding region upstream of ampC and thereby mimics the activated state of AmPR, demonstrating the cross-talk between DNA-binding proteins.

DNA binding proteins

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Overlapping promoters modulate Fnr- and ArcA-dependent anaerobic transcriptional activation of the focApfl operon in Escherichia coli

Article Abstract:

The function of the P6A promoter was analyzed in Escherichia coli gene fusions to determine the interaction between fumarate nitrate reduction factors (Fnr) and the aerobic respiration control regulator (ArcA). Analysis of the physiological function of the Escherichia coli P6A promoter indicated the role of the genetic promoter in modulating Fnr-dependent anaerobic induction. Furthermore, the P6A promoter determines the degree in which ArcA and Fnr transcription factors regulate the expression of the pfl operon.

Physiological aspects, Promoters (Genetics), Microbial respiration

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The bglX gene located at 47.8 min on the Escherichia coli chromosome encodes a periplasmic beta-glucosidase

Article Abstract:

The fourth beta-glucosidase gene (bglX) in Escherichia coli is located in the periplasm at 47.8 min on the chromosome. The enzyme comprises of 745 amino acids and is derived from cleavage of a 20 residue signal peptide. Overproduction or deletion of bglX in E. coli has no effect on phenotype. BglX has a K(sub m) of 18 plus or minus 1mM and a V (sub max) of 3 plus or minus 0.7 micromol min(super 1) for o-nitrophenyl beta-D-glucopyranoside.

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