Genetic and transcriptional organization of the clpC locus in Bifidobacterium breve UCC 2003

Article Abstract:

A homolog of the clpC ATPase gene was identified in the genome of Bifidobacterium breve UCC 2003 by employing a PCR-based approach using primer based on highly conserved regions of ClpC protein in order to identify homologous genes in other bifidobacterial species. A biologically active ClgR molecule requires one or more proteinaceous coactivators to assist in the specific binding of ClgR to the clpC promoter.

Physiological aspects, Protein binding

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Investigation of protein export in Bifodobacterium breve UCC2003

Article Abstract:

An export specific reporter system, designed for use in gram positive organisms and the use of staphylococcal nuclease (Nuc) as a reporter is employed to identify exported proteins in Bifidobacterium breve UCC2003. Conclusions state that proteins exported by Bifidobacterium contain either an SP similar to that of other gram positive bacteria or a number of transmembrane regions.

Membrane proteins, Staphylococcus

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Molecular characterization of hsp20, encoding a small heat shock protein of Bifidobacterium breve UCC2003

Article Abstract:

The genetic characterization of the Bifidobacterium breve UCC2003 hsp20 gene is discussed. Results suggest the strong inducement of transcription of hsp20 in response to severe heat shock regimens and by osmotic shock.

Analysis, Osmotic pressure

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