Genetic relationships among strains of Xanthomonas fragariae based on random amplified polymorphic DNA PCR, repetitive extragenic palindromic PCR, and enterobacterial repetitive intergenic consensus PCR data and generation of multiplexed PCR primers useful for the identification of this phytopathogen

Article Abstract:

The results of random amplified polymorphic DNA (RAPD), repetitive extragenic palindromic (REP) PCR and enterobacterial repetitive intergenic consensus (ERIC) PCR indicate a close relationship between Xanthomonas fragariae strains from different geographic regions. The RAPD, REP and ERIC assays identify nine, four and two genotypes respectively in the X. fragariae isolates. The DNA amplification product patterns and geographic sites of isolation are unrelated. This indicates that the phytopathogenic bacterium has spread through exchange of infected plant germ plasm.

Observations, Genotype, Genotypes, Bacteria, Phytopathogenic, Phytopathogenic bacteria, Microbiological assay

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Use of repetitive (repetitive extragenic palindromic and enterobacterial repetitive intergeneric consensus) sequences and the polymerase chain raction to fingerprint the genomes of Rhizobium meliloti isolates and other soil bacteria

Article Abstract:

Repetitive extragenic palindromic (REP) and enterobacterial repetitive intergeneric consensus (ERIC) polymerase chain reaction (PCR) products of chromosomal DNA from members of the family Rhizobiaceae and the genus Pseudomonas revealed the presence of REP- and ERIC-like sequences that were highly conserved. The REP and ERIC PCR method could be used to distinguish and classify closely related Rhizobium strains.

DNA testing, DNA identification, Rhizobium meliloti

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Use of 16S-23S rRNA intergenic spacer region PCR and repetitive extragenic palindromic PCR analyses of Escherichia coli isolates to identify nonpoint fecal sources

Article Abstract:

The authors have investigated the source-specific Escherichia coli fingerprints as genotypic markers for raw sewage, cows, dogs, gulls and horses. They have evaluated E. coli isolates as tools for identification nonpoint fecal sources via the use of repetitive-PCR and 16S-23S rRNA intergenic spacer region PCR analyses.

Science & research, Belgium, Analysis, Physiological aspects, Environmental aspects, Escherichia coli, Feces, Microbiology, Ribosomal RNA, Genetic markers, Microbial populations, Microbial ecology

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