Genetic relatedness of Bradyrhizobium japonicum field isolates as revealed by repeated sequences and various other characteristics

Article Abstract:

An indigenous field sample of Bradyrhizobium japonicum comprising 49 isolates was analyzed by DNA polymorphisms using four repeated sequence (RS) probes. Cluster analysis of the different RS patterns showed that 44 of isolates could be classified into 33 different RS groups, which were statistically correlated to the hup genotypes. Two other isolates displayed RS patterns markedly different from the patterns of the other isolates. These results demonstrate the usefulness of RS fingerprinting in the analysis of the genetic structure of B. japonicum.

Methods, Usage, Population genetics, Nitrogen-fixing microorganisms

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New Bradyrhizobium japonicum strains that possess high copy numbers of the repeated sequence RS-alpha

Article Abstract:

A study was conducted to examine field isolates of Bradyrhizobium japonicum strains which exhibited numerous bands of RS-specific hybridization. The DNA hybridization profiles of these isolates were analyzed. Results revealed that the highly reiterated sequence-possessing isolates from Bradyrhizobium japonicum showed slower growth and they were derived from normal isolates in individual fields by genome rearrangements which may be mediated by insertion sequence such as RS-alpha.

Analysis, Gram-negative bacteria, Plasmids, Nucleotide sequence, Base sequence, Insertion elements, DNA, DNA insertion elements

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IS1631 occurrence in Bradyrhizobium japonicum highly reiterated sequence-possessing strains with high copy numbers of repeated sequences RSalpha and RSbeta

Article Abstract:

Highly reiterated sequence-possessing (HRS) strains of Bradyrhizobium japonicum have the IS1631 insertion sequence-like element whereas non-HRS strains do not. IS elements are mobile DNA elements that occur in the genomes, plasmids, and bacteriophages of many bacteria.

Research, Transposons, Bacteria, Nitrifying, Nitrobacteraceae

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